The Troponin I Rapid Test Device is a rapid visual immunoassay for the detection of cardiac Troponin I in human whole blood, serum, or plasma specimens. This kit is intended to be used as an aid in the diagnosis of myocardial infarction (MI).
After cardiac injury occurs, Troponin I is released into the blood 4-6 hours after the onset of pain.Troponin I remains elevated for 6-10 days, thus providing for a longer window of detection for cardiac injury.The Troponin I Rapid Test Device has been designed to detect cardiac Troponin I through visual interpretation of color development in the strip. The membrane is immobilized with anti-cTnI antibodies on the test region. During the test, the specimen is allowed to react with colored anti-cTnI antibodies colloidal gold conjugates, which are precoated on the sample pad of the test. The mixture then moves on the membrane by a capillary action, and interact with reagents onthe membrane. If there are enough cTnI in specimens, a colored band will form at the test region of the membrane. Presence of this colored band indicates a positive result.
Add two drops of serum or plasma into the sample window using provided disposable dropper
For Venipuncture Whole Blood specimens: Add 2 drops of whole blood to the specimen well (S), then add 1 drop of Buffer
For Fingerstick Whole Blood: Allow 1 hanging drop of fingerstick whole blood specimen to fall into the center of the specimen well (S), then add 2 drops of buffer
Wait for the colored band(s) to appear. The result should be read at 20 minutes.
Positive: Two lines appear. One coloured line should be in the control line region (C) and another apparent coloured line should be in the test line region (T).
Negative: One coloured line appears in the control line region(C). No line appears in the test line region (T).
Invalid: No visible band at all or test band without control band.