The HIV Triline, Rapid Human Immunodeficiency Virus Test (Serum/Plasma) is a rapid immunoassay for the qualitative detection of Human Immunodeficiency Virus (HIV) type-1 and/or type-2 separately.
The HIV Triline (1/2/0) Rapid Human Immunodeficiency Virus Test is a lateral flow chromatographic immunoassay based on the principle of the double antigen–sandwich technique. The membrane is coated with recombinant HIV 1 and HIV 2 recombinant antigens in the test line region 1 and 2 of the device. When a specimen is applied at one end of the membrane, it reacts with HIV recombinant antigen coated gold conjugate in the test. The mixture then migrates chromatographically by capillary action and reacts with the recombinant HIV recombinant antigens on the membrane in the test line region. If the specimen contains antibodies to HIV-1 or HIV-2, a colored line will appear in the test line region 1 & 2, showing a positive result.
Add 1 drop of serum or plasma into the sample window using provided disposable dropper
Add 2 drops (60-70μl) of buffer
Read results within 15 Minutes. (Do not interpret the result after 20 minutes.)
Positive: Two or three lines appear. One colored line should be in the control line region (C) and another apparent colored line should be test region 1 or 2 or both (1 & 2 printed on the test device)
Negative: One coloured line appears in the control line region(C). No line appears in the test line region (T).
Invalid:No visible band at all or test band without control band.
